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http://dspace.udla.edu.ec/handle/33000/10653| Tipo de material : | bachelorThesis |
| Título : | Estudio de la diversidad genética entre dos poblaciones de anomalocosmoecus illiesi provenientes de los volcanes Cotopaxi e Illinizas |
| Autor : | Cuasapas Arciniega, Samantha Brigitte |
| Tutor : | Ballesteros Redondo, María Isabel |
| Palabras clave : | INFORMACIÓN GEOGRÁFICA;POBLACIÓN;BIODIVERSIDAD |
| Fecha de publicación : | 2019 |
| Editorial : | Quito: Universidad de las Américas, 2019 |
| Citación : | Cuasapaz Arciniega, S. B. (2019). Estudio de la diversidad genética entre dos poblaciones de anomalocosmoecus illiesi provenientes de los volcanes Cotopaxi e Illinizas (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | Anomalocosmoecus illiesi es un insecto macroinvertebrado de agua dulce, correspondiente al orden Trichoptera… |
| Descripción : | Anomalocosmoecus illiesi is a macroinvertebrate fresh water insect, corresponding to the order Trichoptera. Species of this order, including A. illiesi, are used as bioindicators to assess the quality of aquatic ecosystems. Ecuador is a highly biodiverse country, but the knowledge about the species that are in its territory is really poor. Find new information, about the genetic diversity of Anomalocosmoecus illiesi, will help to better understand the diversity patterns of this specie and from other aquatic insects. In this work, we used the DNA barcode, a very useful molecular tool in genetic diversity studies of other Trichoptera species, to infer the evolutionary history among the populations of the Cotopaxi volcano (PI) and the Illinizas volcano (SP). For this purpose, the mitochondrial DNA (mtDNA) of the protein cytochrome c oxidase subunit I (COI) and the ribosomal DNA (rDNA) 28S were used; Additionally, a comparison with the populations of A. illiesi, corresponding to the paramo El Ángel (A) and to the Chimborazo volcano (C) was established. The analyzes were performed on 81 fragments of the 509 bp mtDNA COI gene, because the 28S rDNA showed no variability and was discarded for the phylogenetic analysis. The nucleotide composition showed a higher content of adenine and thymine, and a greater number of transitions than transversions, as is common in insect genomes. The construction of the phylogenetic tree, reflected that there is a relatively high interpopulation variation between C and PI (p same 0.018), and PI and SP (0.016) populations, which represent the highest p values of the study. Concluding that, there may be a geographical barrier between the populations found in the different sides of the Andean mountain range, but a facility of gene flow between the rivers of nearby basins, as in the case of C and SP, whose p-value is the smaller (p same 0.002). Key words: Anomalocosmoecus illiesi, DNA barcode, COI, genetic diversity, geographical barrier |
| URI : | http://dspace.udla.edu.ec/handle/33000/10653 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2019-05.pdf | 6,35 MB | Adobe PDF | Visualizar/Abrir |
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