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http://dspace.udla.edu.ec/handle/33000/10636| Tipo de material : | bachelorThesis |
| Título : | Análisis de la expresión relativa y caracterización del gen que codifica para HSP70 en plántulas de vasconcellea pubescens sometidas a estrés térmico |
| Autor : | Cevallos Vilatuña, Tiffany Yiselle |
| Tutor : | Idrovo Espín, Fabio Marcelo |
| Palabras clave : | TERMODINÁMICA;REACCIONES QUÍMICAS;TEMPERATURA;CULTIVOS |
| Fecha de publicación : | 2018 |
| Editorial : | Quito: Universidad de las Américas, 2018 |
| Citación : | Cevallos Vilatuña, T. Y. (2018). Análisis de la expresión relativa y caracterización del gen que codifica para hsp70 en plántulas de vasconcellea pubescens sometidas a estrés térmico (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | Únicamente el 3.5 por ciento del área de la tierra global no está afectada por algún tipo de factor ambiental. El estrés abiótico impacta de forma significativa al crecimiento y desarrollo de las plantas… |
| Descripción : | Only 3.5 percent of the global land area is not affected by some type of environmental factor. Therefore, abiotic stress significantly impacts the correct growth and development of plants. The increase in temperature due to global warming has negative implications on global crop production systems. However, plants are complex organisms, able to develop tolerance to different stress factors. Taking into account the growing interest arising from this kind of problem, its negative impact on crop fields and therefore the need to find biotechnological tools that enable us to offer a solution, the general objective of this work was to analyze the relative expression and to characterize the gene coding for HSP70 in seedlings of Vasconcellea pubescens subjected to thermal stress. First, seeds of V. pubescens were sown until they reached a suitable height for the trial. From the plants sown, 64 seedlings, distributed among two biological replicates, were subjected to 25 degrees celsius, as control temperature, and to 45 degrees celsius and 55 degrees celsius as stress temperatures, in each case, for 4 hours. The analysis of variance (ANOVA), showed significance for the 3 types of biochemical tests used (electrolyte leak, catalase enzyme activity and proline content). All the tests indicated a thermotolerant response to the thermal stress test with temperatures, considered as extreme. From the RT-PCR products obtained and the gel analysis, a greater expression of the HSP70 gene was observed as the temperature increases. By means of this observation, it was possible to verify that HSP70 responded in V. pubescens in the same way as in the bibliography reported for other plant species. On a similar note, the HSP70 gene over expressed under stress conditions to award the plant tolerance to temperature. Finally, the bioinformatic analysis was carried out with the aim of elucidating, for the first time, a partial sequence of HSP70, as well as its evolutionary history in V. pubescens, an underutilized plant, which could present genes of interest that commercial plants have lost due to domestication. |
| URI : | http://dspace.udla.edu.ec/handle/33000/10636 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2018-38.pdf | 2,63 MB | Adobe PDF | Visualizar/Abrir |
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