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http://dspace.udla.edu.ec/handle/33000/9321| Tipo de material : | bachelorThesis |
| Título : | Frecuencia del polimorfismo -165 G-T (rs1003349) en el gen MMP-14 en individuos de la población ecuatoriana |
| Autor : | Jijón Vergara, Ariana Solange |
| Tutor : | Aguirre Quevedo, Alina |
| Palabras clave : | ALTERACIONES GENÉTICAS;ENFERMEDADES CARDIOVASCULARES;CÁNCER |
| Fecha de publicación : | 2018 |
| Editorial : | Quito: Universidad de las Américas, 2018 |
| Citación : | Jijón Vergara, A. S. (2018). Frecuencia del polimorfismo -165 G/T (rs1003349) en el gen MMP-14 en individuos de la población ecuatoriana (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | Las enfermedades cardiovasculares, el cáncer y las enfermedades pulmonares crónicas son las que más defunciones provocan mundialmente... |
| Descripción : | Cardiovascular diseases, cancer and chronic lung diseases are the leading death causes worldwide. For this reason, several investigations currently are being carried out focus on the study of genetic alterations that occur during the development of these pathologies. During these investigations, matrix metalloproteinases (MMPs) have aroused great interest, since they play a fundamental role in various pathological processes. MMP-14 is a metalloproteinase that is part of the basal membranes, participates in proteolytic events and can activate other proMMPs. Several studies have established that the presence of certain polymorphisms located in the promoter of the MMP-14 gene can influence the expression of this metalloproteinase. These changes in expression could be related to the development of the aforementioned pathologies. Among the studied polymorphisms stands -165G-T. The aim of the study was to determine the genotypic and allelic frequencies of this polymorphism in the Ecuadorian population and compare our results with those obtained from other populations. To accomplish this, we analyzed DNA extracted from peripheral blood of 103 healthy individuals. The genotypic analysis was carried out by polymerase chain reaction and Sanger type sequencing. The bioinformatics analyses of the sequences obtained were performed with the Geneious software. Analysis of the Hardy-Weinberg equilibrium was additionally carried out, and it confirmed that the population was in genetic equilibrium. Genotype (G-T) was found to be the most frequent in the studied population, exhibiting a 58.25 percent prevalence, followed by genotype (T-T) and genotype (G-G), the latter showing the lowest frequency. It was therefore concluded that genotype (G-G) is the rarest homozygote in the study population. It is proposed that these differences in genotypic distribution may be due to ethnic differences between populations or genetic variations in the Ecuadorian population caused by inbreeding. Based on the obtained results and an extended search of relative bibliography, it is suggested that (G-T) genotype may have a protective role in the development of some relevant pathologies. We propose that a similar study be performed in patients with pathologies of interest and to investigate the expression levels of MMP-14 depending on the genotype. |
| URI : | http://dspace.udla.edu.ec/handle/33000/9321 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2018-19.pdf | 1,78 MB | Adobe PDF | Visualizar/Abrir |
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