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dc.contributor.advisorAguirre Quevedo, Alina-
dc.creatorPrado Gaibor, Gabriela Alejandra-
dc.date.accessioned2018-02-02T18:40:25Z-
dc.date.available2018-02-02T18:40:25Z-
dc.date.issued2017-
dc.identifier.citationPrado Gaibor, G. A. (2017). Diagnóstico molecular de áscaris SPP. En niños ecuatorianos en edad escolar (Tesis de pregrado). Universidad de las Américas, Quito.es_ES
dc.identifier.otherUDLA-EC-TIB-2017-42-
dc.identifier.urihttp://dspace.udla.edu.ec/handle/33000/8203-
dc.descriptionAscaris spp. is a genus of ascaridia nematodes of the Ascarididae family, which causes ascariasis in humans and pigs. As for its prevalence, it is closely related to demographic, climatic and socioeconomic phenomena; taking into account that the population that has the highest risk of suffering from the disease is that which is in areas where sanitation and hygiene are deficient. The population that is most affected are school-age children, because they tend to be more active in the infected environment and rarely engage in good health behaviors. As for the diagnosis of Ascaris spp., it is usually detected using conventional methods such as microscopy, but it has low sensitivity and in many cases it is necessary to make a careful preparation for its detection. However Molecular Biology currently offers sensitive and specific techniques for the diagnosis of parasites such as polymerase chain reaction (PCR). In the present study, 580 samples (feces) of school-age children divided into three regions: Costa (n equal to 140), Sierra (n equal to 100) and East (n equal to 340). We analyze the samples through the amplification of the cytochrome c oxidase gene subunit 1 (COX1) for the diagnosis of Ascaris spp. considering as a positive result the presence of a band of 192 bp. For the analysis, DNA extracted from the pure parasite of Ascaris spp was used as a positive control. As a final result, 29 positive samples and 551 negative samples were obtained, which resulted in a prevalence rate of 5 percent of Ascaris spp. in the three regions studied, while the prevalence rate found by microscopy was 3 percent. From the obtained results it can be affirmed that the end-point PCR is specific and sensitive for the diagnosis of Ascaris spp. Finally, the Costa region presented the highest prevalence of the parasite, followed by the East and the Sierra. This may be due to the existence of environmental, socioeconomic and hygiene habits of the population in Costa region that increases the probability of being infected by Ascaris spp. Keywords: Ascaris spp., Risk Factors, Prevalence, PCR, COX1en
dc.description.abstractAscaris spp. Se trata de un género de nemátodos ascaridios de la familia Ascarididae, causante de la ascariasis en humanos y cerdos...es_ES
dc.format.extent87 P.es_ES
dc.language.isospaes_ES
dc.publisherQuito: Universidad de las Américas, 2017es_ES
dc.rightsopenAccesses_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Ecuador*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/ec/*
dc.subjectDIAGNÓSTICO DE LABORATORIOes_ES
dc.subjectENFERMEDADES PARASITARIASes_ES
dc.subjectASCARIASISes_ES
dc.subjectNIÑOSes_ES
dc.titleDiagnóstico molecular de áscaris SPP. en niños ecuatorianos en edad escolares_ES
dc.typebachelorThesises_ES
Aparece en las colecciones: Ingeniería en Biotecnología

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