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http://dspace.udla.edu.ec/handle/33000/10655| Tipo de material : | bachelorThesis |
| Título : | Evaluación de la callogénesis como alternativa de propagación in vitro del mortiño (vaccinium floribundum kunth) |
| Autor : | Egas Castro, Fabricio Xavier |
| Tutor : | Vásquez Castillo, Wilson Arturo |
| Palabras clave : | PROTEINAS VEGETALES;CULTIVOS ORGÁNICOS;HORMONAS |
| Fecha de publicación : | 2019 |
| Editorial : | Quito: Universidad de las Américas, 2019 |
| Citación : | Egas Castro, F. X. (2019). Evaluación de la callogénesis como alternativa de propagación in vitro del mortiño (vaccinium floribundum kunth) (Tesis de pregrado) Universidad de las Américas, Quito. |
| Resumen : | El mortiño Vaccinium floribundum es una baya silvestre que crece en los páramos ecuatorianos sobre los 3400 msnm, es rico en antioxidantes naturales y muy cotizado por su sabor, propiedades medicinales y nutricionales... |
| Descripción : | Andean berry or mortiño (Vaccinium floribundum) is a wild berry that grows in the Ecuadorian moors above 3400 masl, it has natural antioxidants and highly valued for its taste, medicinal and nutritional properties. At present, there are no domesticated crops or efficient propagation methods to produce plants, due to the limitations for their establishment. In the present study, an alternative was established for mortiño micropropagation, for this, the response of plant material to callogenesis was evaluated, in different media, with the use of growth regulators in different concentrations, and submitted to different photoperiods. The induction of calluses of mortiño was carried out evaluating 12 treatments with 5 repetitions, product of the combination of three factors: 1) culture media (Murashige and Skoog-MS 0.5X and Woody Plant Medium-WPM), 2) hormones (Thidiazuron- TDZ, Zeatina-ZEA and Trans-Zeatina-TZR and 3) high dose (TDZ 3 ppm, ZEA and TZR 4 ppm) and low dose (TDZ 1.5 ppm, ZEA and TZR 2 ppm). Four leaves were planted in a petri dish (5cm) incubated in controlled relative humidity and two photoperiods (8h dark / 16h light and 24h dark). The variables considered were oxidation, death of the explants, presence or absence of calluses and percentage of callus formation. The evaluation was every 10 days for a period of 60 days. From the results the best means for callus production was WPM since there was less oxidation and death of the explants. The hormone with the highest percentage of calluses was with TDZ in high doses. From the study it can be inferred that the callogenesis is a viable alternative for the in vitro propagation of mortiño. However, success will depend on the dose of hormones, the culture medium, photoperiod and, finally, the genotype of the material. |
| URI : | http://dspace.udla.edu.ec/handle/33000/10655 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2019-11.pdf | 2,62 MB | Adobe PDF | Visualizar/Abrir |
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