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http://dspace.udla.edu.ec/handle/33000/12246| Tipo de material : | bachelorThesis |
| Título : | Evaluación de tres vías de regeneración in vitro de dos clones promisorios de piñón (Jatropha curcas l.) del banco de germoplasma del INIAP |
| Autor : | Navarro Hinojosa, Alejandro Germán |
| Tutor : | Racines Oliva, Mauricio Andrés |
| Palabras clave : | REPRODUCCIÓN ASISTIDA;FECUNDACIÓN IN-VITRO;BIOTECNOLOGÍA VEGETAL;EMBRIOGÉNESIS SOMÁTICA |
| Fecha de publicación : | 2020 |
| Editorial : | Quito: Universidad de las Américas, 2020 |
| Citación : | Navarro, A. (2020). Evaluación de tres vías de regeneración in vitro de dos clones promisorios de piñón (Jatropha curcas l.) del banco de germoplasma del INIAP (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | El piñón “Jatropha curcas L.” es una planta perenne perteneciente a la familia Euphorbiaceae. Es originaria de Centroamérica y México, actualmente se cultiva en algunas zonas de Asia, África y América Latina. J. curcas ha despertado un gran interés científico debido a su extraordinaria capacidad para adaptarse a climas semiáridos, su alta resistencia a sequías y a suelos salinos... |
| Descripción : | The pine nut (Jatropha curcas L.) is a perennial plant belonging to the family Euphorbiaceae. It is native to Central America and Mexico, currently grown in some areas of Asia, Africa and Latin America. J. curcas has aroused great scientific interest due to its extraordinary capacity for semi-arid climates, its high resistance to droughts and saline soils. In addition, the seeds that produce the pine nut contain a high content of inedible vegetable oils. These oils can be used for the production of synthetic fuels such as biodiesel. In Ecuador, the pine nut is sown by traditional techniques, by means of stakes or seeds, these routes being insufficient for the massive generation of plants and through the production of biodiesel. Due to the poor propagation and lack of knowledge about the genetic diversity of this species, the possibility of generating improved materials has been conditioned, so that a highly productive pine nut variety is not yet known. In this investigation, three in vitro culture regeneration pathways of two promising clones of J. curcas of INIAP were evaluated: CP041 and CP052. The test consists of growing samples of leaves and petioles of these genotypes in different culture media to induce the formation of calluses, somatic embryos and adventitious organs. The results that the Cp052 genotype cultivates in the MICJ-2 medium, which is supplemented with IAA and BAP, the most optimal treatment to induce the formation of calluses and adventitious buds. On the other hand, the results obtained from the somatic embryogenesis phase show that this regeneration path was inefficient. Finally, samples of the Cp041 genotype are grown in MIBJ-2 which contained copper sulfate, IBA and TDZ and are the most optimal treatment for induction of direct adventitious organogénesis. |
| URI : | http://dspace.udla.edu.ec/handle/33000/12246 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2020-13.pdf | 2,04 MB | Adobe PDF | Visualizar/Abrir |
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