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Tipo de material : bachelorThesis
Título : Aislamiento e identificación de microorganismos con capacidad de degradar hidrocarburos en suelos contaminados de la comunidad Lumbaqui, provincia de Sucumbíos
Autor : Naranjo González, Daniela Carolina
Tutor : Bastidas Caldés, Carlos Andrés
Fecha de publicación : 2017
Editorial : Quito: Universidad de las Américas, 2017
Citación : Naranjo González, D. C. (2017). Aislamiento e identificación de microorganismos con capacidad de degradar hidrocarburos en suelos contaminados de la comunidad Lumbaqui, provincia de Sucumbíos (Tesis de pregrado). Universidad de las Américas, Quito.
Resumen : A lo largo de la historia, el petróleo como hidrocarburo y como producto del proceso de refinación, ha generado alto impacto en el ambiente...
Descripción : Throughout history, oil as a hydrocarbon and as a product of the refining process, has generated high impact on the environment. Ecuador is one of the countries with the highest environmental contamination by hydrocarbons, where the Amazon provinces are the most affected. Therefore, it is imperative to develop alternative treatment of contaminated soils that are sustainable, noninvasive or harmful to nature and especially efficient, such as bioremediation. For the present project soils contaminated with hydrocarbons of the Community of Lumbaqui were used, in order to isolate autochthonous bacteria with capacity to degrade hydrocarbons. Under controlled laboratory conditions, the bacterial growth dynamics, the kinetics of the hydrocarbon degradation process and the species tested were identified. These parameters were studied in 2 stages; a turbidity test where bacterial colonies with the highest growth rate yields were selected and a CO2 emission test where the growth kinetics and the rate of hydrocarbon degradation were established by the indirect method of t phenolphthalein’s coloration. From the tests carried out, where White Fuel (Jet Fuel) was used, it was possible to isolate and identify 3 different bacteria capable of degrading this hydrocarbon, Pseudomonas oleovorans, Bacillus sphaericus and Bacillus smithii. According to the data obtained it was determined that P. oleovorans presents a higher growth rate with respect to B.smithii and B.sphaericus with 20% and 53% more, respectively. It was also observed that P.oleovorans and B.smithii have a more efficient degradation rate at 21 days (100% degradation) than B. sphaericus (94% degradation). These observed differences did not show statistical significance. Finally, it was concluded that the bacterial strains isolated in Lumbaqui are able to grow in a substrate contaminated with hydrocarbon and to degrade it efficiently. In addition, it is advisable to carry out studies with other types of hydrocarbons, analyzing the tolerance, degradation efficiency and behavior in microbial consortium of the identified bacteria.
URI : http://dspace.udla.edu.ec/handle/33000/7458
Aparece en las colecciones: Ingeniería en Biotecnología

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