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http://dspace.udla.edu.ec/handle/33000/12682| Tipo de material : | bachelorThesis |
| Título : | Caracterización molecular de la variabilidad genética de la colección de cacao nacional centenario del INIAP-EELS |
| Autor : | Córdova Mariño, Carla Vanessa |
| Tutor : | Morillo Velasteguí, Luis Eduardo |
| Palabras clave : | PROPIEDADES BIOLÓGICAS;GENÉTICA MOLECULAR;CACAO |
| Fecha de publicación : | 2020 |
| Editorial : | Quito: Universidad de las Américas, 2020 |
| Citación : | Córdova, C. (2020). Caracterización molecular de la variabilidad genética de la colección de cacao nacional centenario del INIAP-EELS (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | El Ecuador se destaca internacionalmente como el principal productor y exportador de cacao Nacional o también llamado fino y de aroma, el cual posee características distintivas de aroma y sabor… |
| Descripción : | Ecuador stands out internationally as the main producer and exporter of Nacional cocoa or also called fino y de aroma, which has distinctive characteristics of aroma and flavour. The Nacional cocoa trees with more than 80 years of age that preserve the characteristics of fine flavour cocoa, and that are known as Nacional centenario cocoa have gradually disappeared as they are replaced by hybrid samples, which have been the product of introgression of foreign cocoa germplasm, causing an increase in its genetic variability and a decrease in its distinctive characteristics. Another reason why this crop has been lost is because cocoa collections invariably present samples with inconsistent or repeated identities, which makes their identification and estimation of their genetic variability difficult, and that is why the study of genetic diversity in cocoa crops in Ecuador prevents the total loss of National variety. In this sense, the present work aimed to evaluate the genetic variability of the Nacional centenario cocoa collection (CCNC) of the EELS of INIAP. A total of 243 samples were analyzed with 20 microsatellite markers, using M13 tailing technology. The 10 pairs of duplex amplified microsatellite markers were highly discriminative, generating a total of 109 alleles, with an average of 5.5 alleles per locus. 0.8% of duplicate samples were identified. The average genetic diversity obtained was 0.447 and the polymorphic content index was 0.414, values that demonstrate high genetic diversity. Clustering analysis, principal coordinate analysis, and population allocation analysis demonstrated that the samples are classified into two subpopulations (GN and GM) differentiated by their level of heterozygosity, with a fixation index value of 0.105. The results obtained also showed that microsatellite markers and statistical tools provide enough information to help the management and conservation of cocoa collections, because they allow the identification of representative samples and also establish alternatives for the improvement of this crop. |
| URI : | http://dspace.udla.edu.ec/handle/33000/12682 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
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