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Tipo de material : bachelorThesis
Título : Detección molecular de Entamoeba spp. en poblaciones infantiles de la costa, sierra y oriente del ecuador entre los 10 y 14 años de edad mediante reacción en cadena de la polimerasa
Autor : Muñoz Tapia, Daniela Alejandra
Tutor : Idrovo Espín, Fabio Marcelo
Palabras clave : DETECCIÓN DE ENFERMEDADES;INFECCIONES BACTERIANAS;CULTIVO MOLECULAR;REACCIÓN EN CADENA DE LA POLIMERASA;NIÑOS
Fecha de publicación : 2018
Editorial : Quito: Universidad de las Américas, 2018
Citación : Muñoz Tapia, D. A. (2018). Detección molecular de Entamoeba spp. en poblaciones infantiles de la costa, sierra y oriente del ecuador entre los 10 y 14 años de edad mediante reacción en cadena de la polimerasa. (Tesis de pregrado). Universidad de las Américas, Quito.
Resumen : La amebiasis es una enfermedad parasitaria gastrointestinal producida con mayor frecuencia por Entamoeba histolytica, principal protozoario capaz de invadir la mucosa del intestino y provocar infecciones...
Descripción : Amebiasis is a gastrointestinal parasitic disease most frequently produced by Entamoeba histolytica, the main protozoan that can invade the intestinal mucosa and cause infections. About five species of Entamoeba reside in the intestinal lumen as commensals: E. coli, E. dispar, E. moshkovskii, E. polecki and E. hartmanni; of these the first two and E. histolytica are the most prevalent. In Ecuador, there have been epidemiological studies of Entamoeba spp. in rural communities; however, there is still scarce information that reflects the real prevalence of these parasites because the studies have been based mostly on the use of direct microscopy for diagnosis. This methodology with low specificity and sensitivity for the differentiation of morphologically identical species, has allowed the denomination of these parasites as species complex. Currently, the development of molecular techniques allows epidemiological studies of these microorganisms, including techniques based on the detection of their DNA as the polymerase chain reaction (PCR), which have provided valuable information to decipher the prevalence of this genre. The present work aims to determine the presence of Entamoeba spp. in schoolchildren from the three regions of continental Ecuador, by using endpoint PCR based on genderspecific primers as a diagnostic technique in 508 stool samples of boys and girls between 10 and 14 years of age, compared with screening of Entamoeba spp. through direct microscopy. The results showed a 41.5 percent prevalence of Entamoeba spp. in the three Ecuadorian regions where the East maintained 52.6 percent followed by the Sierra with 41.9 percent and the Coast with 26.2 percent. It was identified that the PCR obtained a sensitivity of 55.3 percent and a specificity of 64.3 percent. The analysis of the results concluded that Entamoeba spp. it is highly prevalent in Ecuador. It was also determined that microscopy, reference standard currently used in Ecuador for the detection of Entamoeba spp., did not provide optimal ranges of sensitivity and specificity.
URI : http://dspace.udla.edu.ec/handle/33000/9085
Aparece en las colecciones: Ingeniería en Biotecnología

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