Por favor, use este identificador para citar o enlazar este ítem:
http://dspace.udla.edu.ec/handle/33000/8889| Tipo de material : | bachelorThesis |
| Título : | Implementación de un protocolo para la detección de rabia paralítica en ganado reproductor bovino |
| Autor : | Moreno Reyes, Jonatan Baruc |
| Tutor : | Tapia López, Wilson David |
| Palabras clave : | PATOLOGÍA ANIMAL;DETECCIÓN DE ENFERMEDADES;RABIA;GANADO BOVINO |
| Fecha de publicación : | 2018 |
| Editorial : | Quito: Universidad de las Américas, 2018 |
| Citación : | Moreno Reyes, J. B. (2018). Implementación de un protocolo para la detección de Rabia paralítica en ganado reproductor bovino (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | La rabia paralítica bovina constituye un grave problema para el sector pecuario en el Ecuador, en especial para la ganadería bovina debido a las grandes pérdidas económicas que ocasiona, así como también por el inminente peligro de transmisión para las personas que manejan hatos ganaderos... |
| Descripción : | Paralytic bovine rabies is a serious problem for the livestock sector in Ecuador, especially for cattle farming due to the great economic losses it causes as well as the imminent danger of transmission for people who manage livestock herds. In the country there is no methodology implemented to determine the presence of bovine rabies virus by PCR and only cases of this disease have been reported using direct immunofluorescence (IFD), inoculation in cell cultures and in mice. DIF has some disadvantages because the labeling of subcellular structures is limited to its use in fixed cells since the antibodies are not able to cross the intact membranes of living cells. It can also happen that the proteins of interest are cross-linked to other proteins, which can cause both false positives and false negatives. For the implementation of the PCR technique, positive controls obtained in experimental animals (mice) were used, from which the brain was taken for the extraction of RNA by TRizol. For the amplification of the nucleoprotein N central gene, a PCR was carried out coupled to reverse transcription (RT-PCR) of the RNA followed by the amplification of the cDNA by conventional PCR. The presence of the amplicon of interest was verified by electrophoresis and the sensitivity and specificity were analyzed as well as the optimal reaction conditions were evaluated. The present work allows us to conclude that PCR is a reliable, sensitive and specific molecular technique for the detection of rabies virus. It was also possible to determine that the best reaction conditions were 58 ° C for 1mM banding for MgCl2 and 0.275 μM for the primer concentration, as well as a theoretical sensitivity for a minimum concentration of 1x10-2 ng / μl. The results obtained allow us to have a protocol for the detection of this virus that allows the analysis of a large number of samples in less time and additionally, the development of future epidemiological studies that facilitate the0020identification of the geographical origin of the natural strains. |
| URI : | http://dspace.udla.edu.ec/handle/33000/8889 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2018-06.pdf | 1,52 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons
