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Tipo de material : bachelorThesis
Título : Caracterización microbiológica y citológica del líquido de edema en anfibios de la especie Gastrotheca spp provenientes de cautiverio de la balsa de sapos
Autor : Oleas Paz, Anahí
Tutor : Genoy-Puerto, Elmer Alexander
Palabras clave : ANIMALES EN PELIGRO DE EXTINCIÓN;ANFÍBIOS;RANAS;FISIOLOGÍA ANIMAL
Fecha de publicación : 2018
Editorial : Quito: Universidad de las Américas, 2018
Citación : Oleas Paz, A. (2018). Caracterización microbiológica y citológica del líquido de edema en anfibios de la especie Gastrotheca spp provenientes de cautiverio de la balsa de sapos (Tesis de pregrado). Universidad de las Américas, Quito.
Resumen : Existe una alta prevalencia de edema en ranas de cautiverio, incluyendo individuos endémicos del Ecuador del género Gastrotheca, los cuales presentan diferentes grados de extinción...
Descripción : There is a high prevalence of edema in captive frogs, including individuals endemic to Ecuador of the genus Gastrotheca, which present different degrees of extinction. Despite this, there is little research that determines the cause of this syndrome, this is due to the difficulty of diagnosis and lack of specific tests for these animals. In the Balsa de Sapos of the Pontificia Universidad Católica del Ecuador, 41 individuals of the genus Gastrotheca were identified who present edema syndrome. The objective of the present study was to characterize at the microbiological, cytological and physicochemical levels the liquid coming from edema of frogs of the genus Gastrotheca spp. of the Balsa de Sapos, to determine possible pathogens present. For which the microbiological presence was determined and identified the pathogens present, main biochemical and cytological components and the composition differences between males and females were determined. Ten males and ten females with edema were sampled from that institution. Morphometric measurements were taken and edema fluid was removed by puncture in the para-medial or paralumbar region. The liquid sample was divided into thirds, the first for the microbiological analysis (bacterial isolation and taxonomic identification), the second for the cytological analysis (cell count and liquid type classification) and the third for the physicochemical analysis (color, appearance, density and pH). A microbiological characterization identified 14 types of bacteria in the edema fluid, which were identified as secondary bacterial infection. In turn, the most frequently identified cells were lymphocytes with 60.1 percent, erythrocytes with 29.3 percent and neutrophils with 4.54 percent. The cytological and physicochemical characterization tests showed that the swelling fluid samples were 35 percent nonseptic exudate, 40 percent septic exudate and the remaining 25 percent transudate. Finally, no relationship was found between the data obtained and the sex of the individuals studied. The data obtained should be analyzed in conjunction with other laboratory tests to have a more accurate diagnosis of the cause of edema in the animals studied.
URI : http://dspace.udla.edu.ec/handle/33000/8752
Aparece en las colecciones: Medicina Veterinaria y Zootecnia

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