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http://dspace.udla.edu.ec/handle/33000/8203| Tipo de material : | bachelorThesis |
| Título : | Diagnóstico molecular de áscaris SPP. en niños ecuatorianos en edad escolar |
| Autor : | Prado Gaibor, Gabriela Alejandra |
| Tutor : | Aguirre Quevedo, Alina |
| Palabras clave : | DIAGNÓSTICO DE LABORATORIO;ENFERMEDADES PARASITARIAS;ASCARIASIS;NIÑOS |
| Fecha de publicación : | 2017 |
| Editorial : | Quito: Universidad de las Américas, 2017 |
| Citación : | Prado Gaibor, G. A. (2017). Diagnóstico molecular de áscaris SPP. En niños ecuatorianos en edad escolar (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | Ascaris spp. Se trata de un género de nemátodos ascaridios de la familia Ascarididae, causante de la ascariasis en humanos y cerdos... |
| Descripción : | Ascaris spp. is a genus of ascaridia nematodes of the Ascarididae family, which causes ascariasis in humans and pigs. As for its prevalence, it is closely related to demographic, climatic and socioeconomic phenomena; taking into account that the population that has the highest risk of suffering from the disease is that which is in areas where sanitation and hygiene are deficient. The population that is most affected are school-age children, because they tend to be more active in the infected environment and rarely engage in good health behaviors. As for the diagnosis of Ascaris spp., it is usually detected using conventional methods such as microscopy, but it has low sensitivity and in many cases it is necessary to make a careful preparation for its detection. However Molecular Biology currently offers sensitive and specific techniques for the diagnosis of parasites such as polymerase chain reaction (PCR). In the present study, 580 samples (feces) of school-age children divided into three regions: Costa (n equal to 140), Sierra (n equal to 100) and East (n equal to 340). We analyze the samples through the amplification of the cytochrome c oxidase gene subunit 1 (COX1) for the diagnosis of Ascaris spp. considering as a positive result the presence of a band of 192 bp. For the analysis, DNA extracted from the pure parasite of Ascaris spp was used as a positive control. As a final result, 29 positive samples and 551 negative samples were obtained, which resulted in a prevalence rate of 5 percent of Ascaris spp. in the three regions studied, while the prevalence rate found by microscopy was 3 percent. From the obtained results it can be affirmed that the end-point PCR is specific and sensitive for the diagnosis of Ascaris spp. Finally, the Costa region presented the highest prevalence of the parasite, followed by the East and the Sierra. This may be due to the existence of environmental, socioeconomic and hygiene habits of the population in Costa region that increases the probability of being infected by Ascaris spp. Keywords: Ascaris spp., Risk Factors, Prevalence, PCR, COX1 |
| URI : | http://dspace.udla.edu.ec/handle/33000/8203 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA- EC- TIB-2017-42.pdf | 2,09 MB | Adobe PDF | Visualizar/Abrir |
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