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http://dspace.udla.edu.ec/handle/33000/7444| Tipo de material : | bachelorThesis |
| Título : | Identificación de fuentes alimentarias de lutzomyia spp. ligadas a casos de leishmaniasis, mediante técnicas moleculares |
| Autor : | Pérez Mejía, Gustavo Eduardo |
| Tutor : | Rojas Silva, Patricio |
| Palabras clave : | ENFERMEDADES PARASITARIAS;ENFERMEDADES VIRALES;SANGRE-ANÁLISIS;ENFERMEDADES-PREVENCIÓN;ECUADOR |
| Fecha de publicación : | 2017 |
| Editorial : | Quito: Universidad de las Américas, 2017 |
| Citación : | Pérez Mejía, G. E. (2017). Identificación de fuentes alimentarias de lutzomyia spp. ligadas a casos de leishmaniasis, mediante técnicas moleculares (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | La leishmaniasis es provocada por protozoarios del género Leishmania, y se transmite por la picadura de vectores dípteros hembras hematófagas, llamados flebótomos del género Phlebotomus en el Viejo Mundo y Lutzomyia en el Nuevo Mundo... |
| Descripción : | Leishmaniasis is caused by protozoan parasites from the genus Leishmania, and it is transmitted by the bite of dipterous female hematophagous vectors, called sand flies belonging to the genus Phlebotomus in the Old World and Lutzomyia in the New World. In the present project we posed the identification of sandflies, their food sources and the presence of parasites of the family Trypanosomatidae in Ecuadorian endemic areas. The sandflies were collected in the locations of Progreso (Guayas), Calceta (Manabí), Shushufindi (Sucumbíos) and Shell (Pastaza) using CDC traps of normal and ultraviolet type light. The taxonomic identification of the collected fed females, by plate mounting in permanent Canada balsam medium and semipermanent Euparal, allowed the identification of 7 genera of sandflies and 8 species of Lutzomyia which were Helcocyrtomyia tortura, Micropygomyia cayennensis, Psychodopygus paraensis, Trichophoromyia Napoensis, Nysomyia yuilli yuilli Psychodopygus davisi, Evandromyia walkeri, and Trichophoromya ubiquitalis. In the case of the H. tortura species, it’s the first time that it is recorded in the Guayas province. The analysis for the identification of dietary sources, by amplification of the mitochondrial cytochrome B gene using the molecular technique of PCR, showed that the highest blood intake corresponds to human blood with a 54%, followed by galliform blood with 26%: there was also recorded the blood ingestion from pig, cow, dog and double ingestion from human and galliform. Likewise, the identification of natural infection by parasites of the family Trypanosomatidae was performed by the amplification of the ITS1 region and by sequencing we were able to identify the presence of Leishmania spp. and L. mexicana. With the results obtained in the collection we concluded that for studies of blood intake in sandflies both traps (of normal light and ultraviolet light) can be used, as in both we collected a similar number of fed females. The results obtained help to complement information on the epidemiological cycle of leishmaniasis in endemic sectors where this type of studies has never been carried out and thus implement control and prevention strategies. |
| URI : | http://dspace.udla.edu.ec/handle/33000/7444 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2017-15.pdf | 1,49 MB | Adobe PDF |  Visualizar/Abrir |
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