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http://dspace.udla.edu.ec/handle/33000/6438Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.advisor | Heredia Moya, Jorge Humberto | - |
| dc.creator | Licto Iler, Shirley Lissete | - |
| dc.date.accessioned | 2017-02-22T15:48:23Z | - |
| dc.date.available | 2017-02-22T15:48:23Z | - |
| dc.date.issued | 2016 | - |
| dc.identifier.citation | Licto Iler, S. L. (2016). Evaluación de la actividad leishmanicida y citotóxica de 4,4'-(arilmetilen)-bis-(1-fenil-3-metil-1h-pirazol-5-oles) frente a leishmania mexicana (Tesis de pregrado). Universidad de las Américas, Quito. | es_ES |
| dc.identifier.other | UDLA-EC-TIB-2016-28 | - |
| dc.identifier.uri | http://dspace.udla.edu.ec/handle/33000/6438 | - |
| dc.description | Leishmaniasis is a disease caused by protozoan parasites of the genus Leishmania and is manifested in three clinical forms: cutaneous, mucocutaneous and visceral. In Ecuador, only the cutaneous form produced by seven species of Leishmania, among which L. mexicana, has been reported. Currently available therapy for leishmaniasis is based on the use of pentavalent antimony compounds that produce serious side effects and also generate resistance in parasites. In this research the leishmanicidal and cytotoxic activity was evaluated of 4,4 '- (arilmetilen) -bis- (1-phenyl-3-methyl-1H-pyrazol-5-ols) against L. mexicana and RAW 264.7 macrophages by the MTT method. The data obtained in this research show that all pyrazol-5-oles evaluated present leishmanicidal effect, the best activity was of C8 and C12 with an IC50 of 33.35 μM for C8 and 89,37μM for C12 after 24h of incubation, and they present a CC50 of 101,4μM for C8 and 144.1 μM for C12 after 48h of treatment. By flow cytometry using the fluorophore DiBAC4 (3) was observed that C8 at a concentration of 150 μM affects membrane potential parasite, while C12 does not affect even at a concentration of 300 μM. An analysis of the structureactivity relationship of the active compounds was performed and the importance of the presence of the nitro group in the ring was observed. | en |
| dc.description.abstract | La leishmaniasis es una enfermedad causada por parásitos protozoarios del género Leishmania y se manifiesta de tres formas clínicas: cutánea, mucocutánea y visceral... | es_ES |
| dc.format.extent | 57 p. | es_ES |
| dc.language.iso | spa | es_ES |
| dc.publisher | Quito: Universidad de las Américas, 2016 | es_ES |
| dc.rights | openAccess | es_ES |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ | es_ES |
| dc.subject | BIOTECNOLOGÍA | es_ES |
| dc.subject | LEISHMANIA | es_ES |
| dc.subject | CITOTOXINAS | es_ES |
| dc.title | Evaluación de la actividad leishmanicida y citotóxica de 4,4'-(arilmetilen)-bis-(1-fenil-3-metil-1h-pirazol-5-oles) frente a leishmania mexicana | es_ES |
| dc.type | bachelorThesis | es_ES |
| Aparece en las colecciones: | Ingeniería en Biotecnología | |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2016-28.pdf | 1,95 MB | Adobe PDF |  Visualizar/Abrir |
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