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http://dspace.udla.edu.ec/handle/33000/1192Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.advisor | Morillo Velasteguí, Luis Eduardo | - |
| dc.creator | Lascano Valarezo, Luis Felipe | - |
| dc.date.accessioned | 2015-06-12T02:36:26Z | - |
| dc.date.available | 2015-06-12T02:36:26Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.citation | Lascano, L. (2020). Análisis de la variabilidad genética de la colección del piñón (Jatropha curcas l.) del INIAP con marcadores SSR-EST (Tesis de pregrado). Universidad de las Américas, Quito. | es_ES |
| dc.identifier.other | UDLA-EC-TIB-2020-16 | - |
| dc.identifier.uri | http://dspace.udla.edu.ec/handle/33000/1192 | - |
| dc.description | Jatropha curcas commonly known as pine nut is a plant of the Euphorbiaceae family, which has a high percentage of oil in its seeds. Its properties attract attention for the production of biodiesel. Since it is not domesticated, both breeding and breeding programs are very limited. A solid genetic base is necessary, so it is essential to obtain more information that characterizes the accessions and provides important information. The sequences SSR (Simple Sequence Repeats), STR (Short Tandem Repeats) or microsatellites, are commonly found in the eukaryotic genome and in certain prokaryotes. Its form of repetition and its frequency in the genome is different in each individual of a species due to its high mutability, so they are highly recommended to genetically characterize plants. In the present study, the genotyping of 163 accessions of the INIAP sprocket collection was performed, using 22 EST-SSR microsatellites and the M13-tailing methodology. A genotypic matrix was obtained, from which different statistical, structure and genetic diversity analyzes were performed. The average expected heterozygous and heterozygous observed were (0.319) and (0.230) respectively, indicating a high level of inbreeding. The average polymorphic information content index (PIC) in all samples was (0.257). A total of 16 similar accessions were identified in the parity analysis, the majority coming from Manabí. The structure analysis divided the accessions studied into two populations G1 and G2, together with the presence of intermediate simples between the two populations. The PCoA analysis showed that the G1 population was very dispersed, with a larger number of samples, in the UPGMA dendrogram it was not possible to define clear subgroups and the bootstraps values were not greater than 50 mostly. The G2 population was less dispersed with fewer samples, no subgroups defined in the dendrogram and also had low Bootstrap values. A second study with PCoA analysis, UPGMA dendrogram and AMOVA was carried out with the highest and lowest Q samples (Estimated membership for each individual in a population). The results of the PCoA better grouped the pinion accessions in the two populations, removing the intermediate accessions that were before. It was possible to identify subgroups in both populations in the UPGMA dendrogram, despite this, bootstraps values remained very low. The AMOVA indicated that there is greater variability within the populations and les among them. Fis and Fit were positive with quite high values both indicating a high level of monogamy in the pinion accessions. | en |
| dc.description.abstract | Jatropha curcas comúnmente conocido como piñón es una planta de la familia Euphorbiaceae, que tiene un alto porcentaje de aceite en sus semillas... | es_ES |
| dc.format.extent | 100 p. | es_ES |
| dc.language.iso | spa | es_ES |
| dc.publisher | Quito: Universidad de las Américas, 2020 | es_ES |
| dc.rights | openAccess | es_ES |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ | es_ES |
| dc.subject | GENÉTICA | es_ES |
| dc.subject | DIVERSIDAD GENÉTICA | es_ES |
| dc.subject | PIÑÓN | es_ES |
| dc.title | Análisis de la variabilidad genética de la colección del piñón (Jatropha curcas l.) del INIAP con marcadores SSR-EST | es_ES |
| dc.type | bachelorThesis | es_ES |
| Aparece en las colecciones: | Ingeniería en Biotecnología | |
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|---|---|---|---|---|
| UDLA-EC-TIB-2020-16.pdf | 2,46 MB | Adobe PDF | Visualizar/Abrir |
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