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dc.contributor.advisorBallesteros Redondo, María Isabel-
dc.creatorLoachamín Punina, Gisella Noemí-
dc.date.accessioned2019-09-18T16:17:47Z-
dc.date.available2019-09-18T16:17:47Z-
dc.date.issued2019-
dc.identifier.citationLoachamín Punina, G. N. (2019). Evaluación de la diversidad genética entre poblaciones de Metrichia de la cordillera andina ecuatoriana (Tesis de pregrado). Universidad de las Américas, Quito.es_ES
dc.identifier.otherUDLA-EC-TIB-2019-24-
dc.identifier.urihttp://dspace.udla.edu.ec/handle/33000/11445-
dc.descriptionThe ideal functioning of ecosystems is directly related to the conservation of species, therefore, the study of their diversity is a priority for researchers. In Ecuador, previous studies on Trichoptera, specifically in the Anomalocosmoecus illiesi species, showed a relatively high interpopulation variation among individuals, generating a guideline for the study of other species belonging to this order, which are used as bioindicators to assess the quality of Aquatic ecosystems In this way, the genus Metrichia was proposed, because of its small size it is cataloged in cryptic diversity, therefore, the phenotypic study must be complemented. For this work the use of phylogeography was proposed, which based on DNA sequences and geographic distribution can interpret the relationship between populations or closely related species. To infer the diversity of genes among populations, mitochondrial DNA from the cytochrome c oxidase subunit I (IOC) protein and 16S ribosomal DNA (rDNA) was used in samples taken from the Illinizas, Chimborazo and Park volcano basins Ecological boxes. The analyzes were performed on 36 fragments of the 477 bp COI mtDNA gene and 45 sequences of 400 bp for the 16S gene. The genetic variability of the gender was analyzed through the Kimura model of 2 parameters in the MEGA7 program, which determined that the population had a 21.7% genetic divergence. However, since in Ecuador there are no studies on the species of the genus Metrichia, the Automatic Barcode Gap Discovery (ABGD) program that groups the samples into operational taxonomic units based on the percentage of divergence was used for phylogenetic analysis, thus , 5 experimental taxonomic units (MOTUs) were determined, with which it was concluded that there are populations distributed along the Ecuadorian mountain range, in addition to an ease of gene flow between them through the inter-Andean alley.en
dc.description.abstractEl funcionamiento ideal de los ecosistemas se encuentra directamente relacionado con la conservación de las especies, por lo tanto, el estudio de la diversidad de las mismas es una prioridad para los investigadores...es_ES
dc.format.extent66 p.es_ES
dc.language.isospaes_ES
dc.publisherQuito: Universidad de las Américas, 2019es_ES
dc.rightsopenAccesses_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Ecuador*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/ec/*
dc.subjectGENÉTICA ANIMALes_ES
dc.subjectDIVERSIDAD GENÉTICAes_ES
dc.subjectBIOLOGÍA MOLECULARes_ES
dc.titleEvaluación de la diversidad genética entre poblaciones de Metrichia de la cordillera andina ecuatorianaes_ES
dc.typebachelorThesises_ES
Aparece en las colecciones: Ingeniería en Biotecnología

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