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http://dspace.udla.edu.ec/handle/33000/10882| Tipo de material : | bachelorThesis |
| Título : | Desarrollo in vitro de embriones de vasconcellea pubescens para la generación de plántulas |
| Autor : | Endara Chiriboga, Esmeralda |
| Tutor : | Idrovo Espín, Fabio Marcelo |
| Palabras clave : | BIOTECNOLOGÍA;COMPUESTOS ORGÁNICOS;CULTIVO IN VITRO;PLÁNTULAS;VASCONCELLEA PUBESCENS |
| Fecha de publicación : | 2019 |
| Editorial : | Quito: Universidad de las Américas, 2019 |
| Citación : | Endara Chiriboga, E. (2019). Desarrollo in vitro de embriones de vasconcellea pubescens para la generación de plántulas (Tesis de pregrado). Universidad de las Américas, Quito |
| Resumen : | Vasconcellea es un género que cuenta con 21 especies dentro de la familia de las Caricáceas, 16 de ellas se encuentran distribuidas en las regiones andinas del Ecuador… |
| Descripción : | There are 21 species in the Vasconcellea genus that are within the Caricaceae family, and 16 of these are located among the Andean region of Ecuador. Some of these species are listed in the International Union for Conservation of Nature (IUCN) Red List of Threatened Species, and therefore, it is of the upmost importance to have an in vitro culture protocol which could be used for it’s conservation. The objective of this investigation was to obtain in vitro seedlings of the species V. pubescens through the induction of somatic embryogenesis. The investigation took place in three different phases: induction of somatic embryogenesis, the ripening process of the somatic embryos, and finally the germination from said embryos into seedlings. An essay was carried out where three exposure times (28, 49 and 62 days) were evaluated to the phytoregulator. In this trial the number of proembryos, the number of mature somatic embryos, and lastly the number of leaves which formed in the obtained seedlings were evalulated. A Randomized Block Desing was used, and the results were analyzed through the R-commander statistics tool (Rcmdr). As a result, proembryos were obtained on the 27th day after sowing in all treatments. Nevertheless, the 28 day exposure period treatment displayed a better development, in globular embryo quantity, in the capacity of the seedlings to form roots, and in the capacity to form leaves. In conclusión, the most effective exposure time to the 2,4-D was the 28 day treatment, yet the remaining exposure times (49 and 62 days) cannot be excluded, since they did generate the desired response as well. |
| URI : | http://dspace.udla.edu.ec/handle/33000/10882 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2019-20.pdf | 1,74 MB | Adobe PDF | Visualizar/Abrir |
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