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http://dspace.udla.edu.ec/handle/33000/10881| Tipo de material : | bachelorThesis |
| Título : | Optimización de procesos de formulación de bacillus subtilis por atomización y liofilización |
| Autor : | Morales Barroso, Andrea Estefanía Silva Velarde, Roberto Gabriel |
| Tutor : | Yánez Mendizábal, Viviana del Rocío |
| Palabras clave : | PROCESAMIENTO;LIOFILIZACIÓN;ATOMIZACIÓN;ANÁLISIS BACTERIOLÓGICO;BACILLUS SUBTILIS |
| Fecha de publicación : | 2019 |
| Editorial : | Quito: Universidad de las Américas, 2019 |
| Citación : | Morales Barroso, A. E.; Silva Velarde, R. G. (2019). Optimización de procesos de formulación de bacillus subtilis por atomización y liofilización (Tesis de pregrado). Universidad de las Américas, Quito |
| Resumen : | Investigaciones previas con la bacteria Bacillus subtilis aislado Ctpx S2-1 han demostrado su eficacia para controlar o reducir la antracnosis de chocho Andino (Lupinus mutabilis Sweet) causada por Colletotrichum acutatum durante las fases de campo y en las semillas almacenadas en poscosecha... |
| Descripción : | Previous researches with the bacteria Bacillus subtilis isolated Cptx S2-1 have shown their effectiveness to control or reduce the anthracnose of Andean chocho (Lupinus mutabilis Sweet) caused by Colletotrichum acutatum during the field phases and in the stored seeds in postharvest. At the same time, preliminary studies directed to optimize the production of this bacteria in low cost mean and laboratory scale have been led with the purpose of developing a bio pesticide that serves as an efficient alternative to the chemical treatments used to control the anthracnose of chocho. Based on this, the objective of the present work was to optimize the formulation processes of Bacillus subtilis Ctpx S2-1 by spray drying and freeze drying on a laboratory scale. For this purpose, the concentrations of total cells and endospores produced by the bacteria in a low cost mean and based on corn starch and soy protein studied in previous researches were quantified first. Afterwards, a stuy of the best carriers that maintain the viability of the bacteria was made, as 10% MgSO4, 20% MgSO4, 10% skim milk and 10% MgSO4 + 10% skim milk, which were before and after of the formulation by spray drying and freeze drying. Finally, the influence of the drying process by spray drying and freeze drying on bacterial survival percentages (viability of total cells and endospores in UFC mL-1), recovery of dry powder and relative humidity of formulated products was compared. The concentration bacteria results of the different carriers, fluctuated between 7.8 and 8.2 log UFC mL-1 before the formulation, which demonstrates that these carriers do not affect the survival of the bacteria before formulating. After the formulation process (spray drying and freeze drying) the bacteria concentration were 7.2 and 7.8 log UFC g-1 respectively. This data determines that there are not significant differences between both formulation methods (decrease of 0.6 log UFC). Regarding the carrie the moisture content between 15 and 21% without significant differences. Based on these results, the spray drying compare with the freeze drying can be used as an efficient formulation process for B. subtilis Ctpx S2-1, studying other varieties of carrier concentrations that allow optimize the survival, just like products recovery and low the relative moisture |
| URI : | http://dspace.udla.edu.ec/handle/33000/10881 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2019-19.pdf | 1,88 MB | Adobe PDF | Visualizar/Abrir |
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