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http://dspace.udla.edu.ec/handle/33000/10826| Tipo de material : | bachelorThesis |
| Título : | Análisis de secuencias de marcadores moleculares de introgresión en variedades de origen andino y mesoaméricas de fréjol (Phaseolus vulgaris) |
| Autor : | López Córdova, Paola Denisse |
| Tutor : | Morillo Velasteguí, Luis Eduardo |
| Palabras clave : | LEGUMBRE;PRODUCTOS AGRÍCOLAS;GENÉTICA VEGETAL |
| Fecha de publicación : | 2019 |
| Editorial : | Quito: Universidad de las Américas, 2019 |
| Citación : | López Córdova, P. D. (2019). Análisis de secuencias de marcadores moleculares de introgresión en variedades de origen andino y mesoamericas de fréjol (Phaseolus vulgaris). (Tesis de pregrado). Universidad de las Américas, Quito. |
| Resumen : | El fréjol común (Phaseolus vulgaris L.) o también conocido como: poroto, judía y habichuela, es considerado una de las leguminosas de mayor importancia para el consumo humano... |
| Descripción : | Common bean (Phaseolus vulgaris L.) or also known as bean, bean and snap bean, is considered one of the most important legumes for human consumption. Beans are some source rich in proteins, carbohydrates, vitamins and minerals. In addition, it can be produced in diverse systems of crops and environments, which facilitates it’s being a cosmopolitan specie. In Ecuador, bean production reaches a percentage of 0.2 percent of world production, which corresponds to approximately 5.127 ha of cultivated area. The origin of beans has not been clarified yet. In Mesoamerica and the Andean region, the origin and age of beans have been questioned. Previously conducted studies in Ecuador reported the presence of Mesoamerican alleles in traditional Andean varieties. However, these results are not conclusive due to the techniques used, such as: electrophoresis for the genotyping of micro satellite markers and the size of fragments that hinder the amount of polymorphism. The objective of this study was to analyze the genetic variation of the candidate markers of introgression in varieties of beans of Andean and Mesoamerican origin, for which optimal PCR conditions were established to amplify the allelic range by marker and to define introgressed alleles of interest. The extended sequence microsatellite alleles (SSR) were sequenced. When the alleles were homozygous, they were directly sequenced, and when they were heterozygous, they were cloned. With these data, a phylogenetic analysis was performed for three loci in study: BM 156, BM 160, BM 183, where it was shown that marker BM 160 is the one with the highest polymorphism level in adjoining sequence and the more appropriate to test the hypothesis of introgression This marker clearly differentiated between Mesoamerican and Andean alleles with a statistical robustness level greater than 75-80 percent Key words: Bean (Phaseolus vulgaris), Bean Origin and Domestication, Introgression, microsatellite markers and sequencing |
| URI : | http://dspace.udla.edu.ec/handle/33000/10826 |
| Aparece en las colecciones: | Ingeniería en Biotecnología |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| UDLA-EC-TIB-2019-13.pdf | 2,64 MB | Adobe PDF | Visualizar/Abrir |
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