Por favor, use este identificador para citar o enlazar este ítem: http://dspace.udla.edu.ec/handle/33000/10824
Tipo de material : bachelorThesis
Título : Estandarización de un método rápido de detección del virus del mosaico del tomate (ToMV) mediante reacción en cadena de la polimerasa con transcripción inversa (RT-PCR)
Autor : Ortiz Delgado, Dayanna Sofía
Ramírez Suárez, Ahyme María
Tutor : Rivas Romero, Fernando Xavier
Palabras clave : AGRICULTURA;GENÉTICA;VIROLOGÍA
Fecha de publicación : 2019
Editorial : Quito: Universidad de las Américas, 2019
Citación : Ortiz Delgado, D. S.; Ramírez Suárez, A. M. (2019). Estandarización de un método rápido de detección del virus del mosaico del tomate (ToMV) mediante reacción en cadena de la polimerasa con transcripción inversa (RT-PCR) (Tesis de pregrado). Universidad de las Américas, Quito.
Resumen : El virus del mosaico del tomate “ToMV”, tiene un alto impacto en la agricultura, afectando principalmente a cultivos de la familia Solanaceae...
Descripción : The tomato mosaic virus (ToMV) has a high impact on agriculture, mainly affecting crops of Solanaceae family. The characteristic symptomatology of this virus is the presence of curly shape and yellowish spots in the form of ring in leaves, deformed leaflets and reduced growth of the plant. Until now there is no detection method in the country for this virus, so it is important to develop and implement this method to detect tomato mosaic virus and that can be better controlled. The reverse transcriptase polymerase chain reaction (RT-PCR) method is a molecular tool that allows in a preliminary way and at an early stage the detection of the virus by amplifying the genetic material of the pathogen. The main objective of the project was to develop a rapid method of detection for tomato mosaic virus by RT-PCR. To carry out the objectives of this study, PCR conditions were standardized, such as MgCl2 concentration, hybridization temperature and primer concentration. In this way, a commercial positive control was used, to which the PCR conditions were adjusted and the amplicons were validated by sequencing. As results, MgCl2 concentration of 2 mM, hybridization temperature of 59 degrees Celsius and primer concentration of 0.1 μM were obtained as the most suitable for the RT-PCR reaction. It was determined, by in silico specificity analysis, that the primers used in the study had a high degree of specificity, since they were specific for tomato mosaic virus. The standardized RT-PCR conditions described in the present study, provide a cost-effective, useful, accurate and convenient methodology for its application in research laboratories for the rapid detection of tomato mosaic virus, thus allowing an analysis of this virus in several crops.
URI : http://dspace.udla.edu.ec/handle/33000/10824
Aparece en las colecciones: Ingeniería en Biotecnología

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